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222288 bacterial expression plasmid  (Addgene inc)


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    Addgene inc 222288 bacterial expression plasmid
    222288 Bacterial Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/222288+bacterial+expression+plasmid/pEGFPC1-EPSIN+1+(Plasmid+%2322228)/pmc11996680-267-5-4
    Average 93 stars, based on 13 article reviews
    222288 bacterial expression plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: KIF1C activates and extends dynein movement through the FHF cargo adapter
    Article Snippet: Amino acids 642–922 from human KIF1C were amplified from pKIF1C-2xFlag using AS696 (GAGTCgaattcGAAATGGAGAAGAGGCTGCAG) and AS778 (ATAAATgcggccgcCTACTCCCAGCTTGACAGTGGTG), digested with Eco RI and Not I, and ligated into pGEX-6P2-LTLT to create pGEX-6P2-LTLT-KIF1C(642–922)-6His (Addgene 222289). .. The GST–KIF1C stalk–GFP (pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His, Addgene 222288) bacterial expression plasmid was created by opening pET22b-HsCLASP2-EBBD-eGFP-6His between the NdeI and SalI sites and ligating in a PCR product of GST–KIF1C stalk (previously described) formed between oligonucleotides AS777 (TGCCAcatATGTCCCCTATACTAGGTTATT) and AS780 (ATATTgtcgaccCTCCCAGCTTGACAG). .. All other variants of the GST–KIF1C stalk construct (KIF1CS 2 : 674–922, KIF1CS 3 : 674–822, KIF1CS SGD: 720–841, KIF1C stalk ∆SGD_1: 642–922 ∆722–840, KIF1C stalk ∆SGD_2: 642–922 ∆722–840) were based on the original GST–KIF1C stalk plasmid (Addgene 222288), where fragments were PCR amplified at the indicated sequence positions and assembled using Gibson cloning .

    Article Title: KIF1C activates and extends dynein movement through the FHF cargo adapter.
    Article Snippet: Amino acids 642–922 from human KIF1C were amplified from pKIF1C-2xFlag using AS696 (GAGTCgaattcGAAATGGAGAAGAGGCTGCAG) and AS778 (ATAAATgcggccgcCTACTCCCAGCTTGACAGTGGTG), digested with EcoRI and NotI, and ligated into pGEX-6P2-LTLT to create pGEX6P2-LTLT-KIF1C(642–922)-6His (Addgene 222289). .. The GST–KIF1C stalk–GFP (pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His, Addgene 222288) bacterial expression plasmid was created by opening pET22b-HsCLASP2-EBBD-eGFP-6His between the NdeI and SalI sites and ligating in a PCR product of GST–KIF1C stalk (previously described) formed between oligonucleotides AS777 (TGCCAcatATGTCCCCTATACTAGGTTATT) and AS780 (ATATTgtcgaccCTCCCAGCTTGACAG). .. All other variants of the GST–KIF1C stalk construct (KIF1CS2: 674– 922, KIF1CS3: 674–822, KIF1CS SGD: 720–841, KIF1C stalk ∆SGD_1: 642– 922 ∆722–840, KIF1C stalk ∆SGD_2: 642–922 ∆722–840) were based on the original GST–KIF1C stalk plasmid (Addgene 222288), where fragments were PCR amplified at the indicated sequence positions and assembled using Gibson cloning62.

    Plasmid Preparation:

    Article Title: KIF1C activates and extends dynein movement through the FHF cargo adapter
    Article Snippet: Amino acids 642–922 from human KIF1C were amplified from pKIF1C-2xFlag using AS696 (GAGTCgaattcGAAATGGAGAAGAGGCTGCAG) and AS778 (ATAAATgcggccgcCTACTCCCAGCTTGACAGTGGTG), digested with Eco RI and Not I, and ligated into pGEX-6P2-LTLT to create pGEX-6P2-LTLT-KIF1C(642–922)-6His (Addgene 222289). .. The GST–KIF1C stalk–GFP (pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His, Addgene 222288) bacterial expression plasmid was created by opening pET22b-HsCLASP2-EBBD-eGFP-6His between the NdeI and SalI sites and ligating in a PCR product of GST–KIF1C stalk (previously described) formed between oligonucleotides AS777 (TGCCAcatATGTCCCCTATACTAGGTTATT) and AS780 (ATATTgtcgaccCTCCCAGCTTGACAG). .. All other variants of the GST–KIF1C stalk construct (KIF1CS 2 : 674–922, KIF1CS 3 : 674–822, KIF1CS SGD: 720–841, KIF1C stalk ∆SGD_1: 642–922 ∆722–840, KIF1C stalk ∆SGD_2: 642–922 ∆722–840) were based on the original GST–KIF1C stalk plasmid (Addgene 222288), where fragments were PCR amplified at the indicated sequence positions and assembled using Gibson cloning .

    Article Title: KIF1C activates and extends dynein movement through the FHF cargo adapter.
    Article Snippet: Amino acids 642–922 from human KIF1C were amplified from pKIF1C-2xFlag using AS696 (GAGTCgaattcGAAATGGAGAAGAGGCTGCAG) and AS778 (ATAAATgcggccgcCTACTCCCAGCTTGACAGTGGTG), digested with EcoRI and NotI, and ligated into pGEX-6P2-LTLT to create pGEX6P2-LTLT-KIF1C(642–922)-6His (Addgene 222289). .. The GST–KIF1C stalk–GFP (pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His, Addgene 222288) bacterial expression plasmid was created by opening pET22b-HsCLASP2-EBBD-eGFP-6His between the NdeI and SalI sites and ligating in a PCR product of GST–KIF1C stalk (previously described) formed between oligonucleotides AS777 (TGCCAcatATGTCCCCTATACTAGGTTATT) and AS780 (ATATTgtcgaccCTCCCAGCTTGACAG). .. All other variants of the GST–KIF1C stalk construct (KIF1CS2: 674– 922, KIF1CS3: 674–822, KIF1CS SGD: 720–841, KIF1C stalk ∆SGD_1: 642– 922 ∆722–840, KIF1C stalk ∆SGD_2: 642–922 ∆722–840) were based on the original GST–KIF1C stalk plasmid (Addgene 222288), where fragments were PCR amplified at the indicated sequence positions and assembled using Gibson cloning62.

    Polymerase Chain Reaction:

    Article Title: KIF1C activates and extends dynein movement through the FHF cargo adapter
    Article Snippet: Amino acids 642–922 from human KIF1C were amplified from pKIF1C-2xFlag using AS696 (GAGTCgaattcGAAATGGAGAAGAGGCTGCAG) and AS778 (ATAAATgcggccgcCTACTCCCAGCTTGACAGTGGTG), digested with Eco RI and Not I, and ligated into pGEX-6P2-LTLT to create pGEX-6P2-LTLT-KIF1C(642–922)-6His (Addgene 222289). .. The GST–KIF1C stalk–GFP (pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His, Addgene 222288) bacterial expression plasmid was created by opening pET22b-HsCLASP2-EBBD-eGFP-6His between the NdeI and SalI sites and ligating in a PCR product of GST–KIF1C stalk (previously described) formed between oligonucleotides AS777 (TGCCAcatATGTCCCCTATACTAGGTTATT) and AS780 (ATATTgtcgaccCTCCCAGCTTGACAG). .. All other variants of the GST–KIF1C stalk construct (KIF1CS 2 : 674–922, KIF1CS 3 : 674–822, KIF1CS SGD: 720–841, KIF1C stalk ∆SGD_1: 642–922 ∆722–840, KIF1C stalk ∆SGD_2: 642–922 ∆722–840) were based on the original GST–KIF1C stalk plasmid (Addgene 222288), where fragments were PCR amplified at the indicated sequence positions and assembled using Gibson cloning .

    Article Title: KIF1C activates and extends dynein movement through the FHF cargo adapter.
    Article Snippet: Amino acids 642–922 from human KIF1C were amplified from pKIF1C-2xFlag using AS696 (GAGTCgaattcGAAATGGAGAAGAGGCTGCAG) and AS778 (ATAAATgcggccgcCTACTCCCAGCTTGACAGTGGTG), digested with EcoRI and NotI, and ligated into pGEX-6P2-LTLT to create pGEX6P2-LTLT-KIF1C(642–922)-6His (Addgene 222289). .. The GST–KIF1C stalk–GFP (pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His, Addgene 222288) bacterial expression plasmid was created by opening pET22b-HsCLASP2-EBBD-eGFP-6His between the NdeI and SalI sites and ligating in a PCR product of GST–KIF1C stalk (previously described) formed between oligonucleotides AS777 (TGCCAcatATGTCCCCTATACTAGGTTATT) and AS780 (ATATTgtcgaccCTCCCAGCTTGACAG). .. All other variants of the GST–KIF1C stalk construct (KIF1CS2: 674– 922, KIF1CS3: 674–822, KIF1CS SGD: 720–841, KIF1C stalk ∆SGD_1: 642– 922 ∆722–840, KIF1C stalk ∆SGD_2: 642–922 ∆722–840) were based on the original GST–KIF1C stalk plasmid (Addgene 222288), where fragments were PCR amplified at the indicated sequence positions and assembled using Gibson cloning62.



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    222288 bacterial expression plasmid  (Addgene inc)
    93
    Addgene inc 222288 bacterial expression plasmid
    222288 Bacterial Expression Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/222288+bacterial+expression+plasmid/pEGFPC1-EPSIN+1+(Plasmid+%2322228)/pmc11996680-267-5-4
    Average 93 stars, based on 1 article reviews
    222288 bacterial expression plasmid - by Bioz Stars, 2026-09
    93/100 stars
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